Commercial Allelic Ladders

Each manufacturer of STR kits provides allelic ladders that may be used for accurate genotyping. It is important to note that kits from the Promega Corporation and Applied Biosystems for comparable STR markers often contain different alleles in their allelic ladders. For example, the PowerPlex® 1.1 kit from Promega contains alleles 7-15 in its D5S818 allelic ladder while the Profiler Plus™ kit from Applied Biosystems contains alleles 7-16 in its D5S818 allelic ladder. By having an allele present in the ladder, a laboratory can be more confident of a call from

+

100 125 150 175 200 ■ 1 1 1 1

225 250 275 _1_1_1_

300 325 _1_1_

350 375

800, 600, 400, 200, 0,

D8S1179 D21S11 D7S820 CSF1PO

(12 alleles) JDnll <10 alleles> (10 alleles) (24 alleles)

Blue panel

800 600 400 200 0

___i.uiixa___UlilUl 1

D"?3" D016,S539 D2S1338

(8alleles) (9 alleles) (14 alleles)

M'dkjmujii-jiàyjyijij

Green panel

Ull

(15 alleles) (14 alleles)

.. 111 lllllillihi l,_JülllJ.liUll,L

TPOX

(8 alleles)

(23 alleles)

WlAAJUAÀÛM

Yellow panel

(2 alleles) (10 alleles)

(19 alleles)

iikuMj^oiix/LL

Red panel

(9 alleles)

JAHaji.U

100 bp 139 bP 160 bp 200 bp

1

300 bp 340 bp 300 bp 340 bp

Orange panel 350 bp 1

200 0

1 1 1 1 1

I

1

LIZ-labeled GS500 DNA sizing standard

LIZ-labeled GS500 DNA sizing standard an unknown sample that is being analyzed. In the D5S818 example listed here, one would be more confident typing an observed allele 16 when using the Applied Biosystems kit than the Promega kit because the D5S818 allelic ladder has an allele 16 in the ABI kit. The alleles present in the two sources of commercially available multiplex STR kits are reviewed and contrasted in Table 5.4.

Some of the more recent kits come with an amazing number of alleles in their ladders. For example, the Identifiler™ kit from Applied Biosystems contains 205 alleles (Table 5.4; Figure 5.6). Putting together and mass-producing such a large set of alleles is an impressive feat. The Promega PowerPlex® 16 kit has 209 alleles in its allelic ladders.

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