Different Excitation Laser

The FMBIO II uses a 532 nm laser wavelength to excite fluorescent dyes. The different laser excitation wavelength compared to the typical argon ion laser wavelengths of 488 nm and 514.5 nm means that the two dyes used to label STR alleles in the PowerPlex system are differentially excited causing the dye sensitivities to vary. This effect produces different relative intensities of the amplification products detected in the different instruments. Therefore, the primer concentrations were re-configured to make the amplification products from the PowerPlex systems compatible with the two instruments' different excitation/ emission wavelengths (Lins et al. 1998). Hence, the primer concentrations in the PowerPlex™ 1.2 STR kit has been optimized for use on the ABI 310 while PowerPlex™ 1.1 and 2.1 kits have been designed for the FMBIO II.

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Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

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