Dna Quantities Used In Fluorescencebased Str Typing

PCR amplification is dependent on the quantity of template DNA molecules added to the reaction. Based on the DNA quantitation results obtained using either the slot blot procedure or some other test, the extracted DNA for each sample is adjusted to a level that will work optimally in the PCR amplification reaction. Most commercial STR typing kits work best with an input DNA template of around 1 ng.

A quantity of 1 ng of human genomic DNA corresponds to approximately 333 copies of each locus that will be amplified (D.N.A. Box 3.3). There are approximately 6pg (one million one millionth of a gram or 10-12 grams) of genomic DNA in each cell containing a single diploid copy of the human genome. Thus, a range of typical DNA quantities from 0.1-25 ng would involve approximately 30-8330 copies of every DNA sequence.

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CHAPTER 4

Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

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