Estimating mtDna Quantity

Many laboratories perform a nuclear DNA quantitation assay and then estimate the amount of mtDNA present assuming a fixed ratio between nuclear and mtDNA. For example, 50 or 500 pg of DNA template may be used in an mtDNA amplification based on a nuclear quantification result from Quantiblot (see Chapter 3). Newer approaches involving real-time PCR (see Chapter 4) have begun to appear in the literature (Meissner et al. 2000, Andreasson et al. 2002, von Wurmb-Schwark et al. 2002) that enable direct characterization of the number of mtDNA molecules in a cell.

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Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

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