Many laboratories perform a nuclear DNA quantitation assay and then estimate the amount of mtDNA present assuming a fixed ratio between nuclear and mtDNA. For example, 50 or 500 pg of DNA template may be used in an mtDNA amplification based on a nuclear quantification result from Quantiblot (see Chapter 3). Newer approaches involving real-time PCR (see Chapter 4) have begun to appear in the literature (Meissner et al. 2000, Andreasson et al. 2002, von Wurmb-Schwark et al. 2002) that enable direct characterization of the number of mtDNA molecules in a cell.
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This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.