SRM 2392-I, titled Mitochondrial DNA Sequencing (Human) Standard, was released in June 2003. This SRM is intended to provide quality control when performing the polymerase chain reaction (PCR) and sequencing human mitochondrial DNA (mtDNA) for forensic investigations, medical diagnosis, or mutation detection as well as to serve as a control when PCR amplifying and sequencing any DNA sample.
SRM 2392-I contains extracted DNA from the human cell line HL-60 (65 |L DNA at 1.4ng/|lL) that has been sequenced across the entire mtDNA genome (Levin et al. 2003). A list of 58 unique primer sets that were designed to amplify any portion or the entire human mtDNA genome is also included.
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