The number of samples that may be run on each gel is flexible and depends on the comb used to form the sample loading wells. The Bode Technology Group uses a comb with 38 slots containing flat wells with intervening 'posts' that separate each loading well. Two slots are left empty on each end. Control lanes include a K 562 sample, a reagent blank, a negative control, and a blind control sample for quality control purposes. The remaining 30 lanes are composed of five sets of allelic ladders and 25 test samples. Internal lane standards are loaded into each lane to enable size determination and allele calling of PCR products amplified at each STR locus (shown in blue in Figure 13.11).
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This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.