Several other DNA extraction methods have been used to isolate DNA successfully prior to further sample processing (see Vandenberg et al. 1997). A simple closed tube DNA extraction method has been demonstrated with a thermal stable protease that looks very promising (Moss et al. 2003). Microwave extraction has been used to shorten the conventional organic extraction method by several hours and to yield genomic DNA that could be PCR-amplified (Lee et al. 1994). The addition of 6 M NaCl to a proteinase K-digested cell extract followed by vigorous shaking and centrifugation results in a simple precipitation of the proteins (Miller et al. 1988). The supernatant containing the DNA portion of cell extract can then be added to a PCR reaction. A simple alkaline lysis with 0.2 M NaOH for five minutes at room temperature has been shown to work as well (Rudbeck and Dissing 1998, Klintschar and Neuhuber 2000).
While each of these methods is effective for extracting most DNA samples, the forensic community has traditionally relied on the three main methods mentioned: organic extraction, Chelex extraction, and FTA paper. Higher-throughput approaches, though, are incorporating 96-well extraction formats with products such as the QIAamp® 96 DNA Blood Kit (Holland et al. 2003). The typical amounts of DNA that may be recovered from various biological materials are listed in Table 3.2.
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This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.