PCR amplification of mtDNA is usually done with 34-38 cycles. Protocols for highly degraded DNA specimens even call for 42 cycles (Gabriel et al. 2001a). Sometimes excess Taq is added to overcome PCR inhibitors such as melanin (Wilson et al. 1995a). It is important to keep in mind that sensitivity is maximized with mtDNA testing as it is usually only turned to as a last resort in efforts to obtain DNA results from a sample. The higher the sensitivity of any assay, the greater the chance for contamination and thus greater care is usually required with mtDNA work than with conventional STR typing.
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This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.