Pcrbased Testing Standard Srm 2391b

SRM 2391b, titled PCR-based DNA Profiling Standard, was re-issued in 2002. It is an update of the original SRM 2391 that became available in 1995 and includes certified values for new STR loci. SRM 2391b is intended for use in standardizing forensic and paternity testing quality assurance procedures involving polymerase chain reaction (PCR)-based genetic testing as well as instructional law enforcement and non-clinical research purposes.

It contains 12 components of well-characterized DNA in two forms: genomic DNA and DNA to be extracted from cells spotted on filter paper. There are 10 genomic DNA samples all at a concentration of 1 ng/|L (20 ||L volume). Cell lines 9947A and 9948 are included on a 6 mm Schleicher & Schuell 903 filter paper circle spotted with 5 X 104 cells. The cells permit a laboratory to test its ability to perform DNA extraction while the genomic DNA materials may be used to verify reliable PCR amplification and detection technologies.

Certified genotype values for the 12 SRM components are listed for the FBI's CODIS 13 STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and VWA) as well as additional STR loci F13A01, F13B, FES/FPS, LPL, Penta D, Penta E, D2S1338, D19S433, and SE33. These STR markers are all available in commercial kits from either the Promega Corporation or Applied Biosystems. Certified values for the genetic loci HLA-DQA1, PolyMarker, D1S80, and amelogenin are also described for the 12 components.

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