Realtime Pcr Approaches To Dna Quantification

The primary purpose in performing a DNA quantification test is to determine the amount of 'amplifiable' DNA. A PCR amplification reaction may fail due to the presence of co-extracted inhibitors, highly degraded DNA, insufficient DNA quantity, or a combination of all of these factors. Thus, a test that can accurately reflect both the quality and the quantity of the DNA template present in an extracted sample is beneficial to making decisions about how to proceed. 'Realtime' PCR assays provide such an assessment. The theory behind real-time PCR and assay formats used will be discussed in more detail as part of Chapter 4.

Several real-time PCR assay have been published with the human identity testing market in mind (see Table 3.3). Commercial kits for detecting human DNA as well as a real-time PCR assay for determining the amount of human Y-chromosome DNA present in a sample are now available (Applied Biosystems 2003).

Was this article helpful?

0 0
Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

Get My Free Ebook

Post a comment