Rflp Testing Standard Srm 2390

SRM 2390, titled DNA Profiling Standard (RFLP-based typing methods), was released in August 1992 and is intended for use in standardizing forensic and paternity testing quality assurance procedures for restriction fragment length polymorphism (RFLP) testing that uses HaeIII restriction enzymes as well as instructional law enforcement and non-clinical research purposes.

It contains two well-characterized human DNA samples: a female cell line (K562) and a male source (TAW). Both samples are available in three forms: as a cell pellet (3 X 106 cells), an extracted genomic DNA (~200ng/|lL), and a HaeIII restriction digest (pre-cut DNA; 25ng/|lL). A molecular weight marker for DNA sizing purposes and six quantitation standards (250 ng, 100 ng, 50 ng, 25 ng, 12.5 ng and 6ng) are also included as is agarose for slab gel preparation.

Certified values for the DNA band sizes are available for five commonly used RFLP markers. These markers (and VNTR probes) are D2S44 (YNH24), D4S139 (PH30), D10S28 (TBQ7), D1S7 (MS1), and D17S79 (V1). The certified values represent the pooled results from analyses performed at NIST and 28 collaborating laboratories and come with calculated uncertainties (see Duewer et al. 2000).

Was this article helpful?

0 0
Stammering Its Cause and Its Cure

Stammering Its Cause and Its Cure

This book discusses the futility of curing stammering by common means. It traces various attempts at curing stammering in the past and how wasteful these attempt were, until he discovered a simple program to cure it. The book presents the life of Benjamin Nathaniel Bogue and his struggles with the handicap. Bogue devotes a great deal of text to explain the handicap of stammering, its effects on the body and psychology of the sufferer, and its cure.

Get My Free Ebook

Post a comment