Solution For 13 Codis Str Loci

The Promega solution to examining the 13 CODIS STR loci with the FMBIO instrument platform involves either the use of PowerPlex® 1.1 and 2.1 STR kits or a single megaplex amplification with PowerPlex® 16 BIO (see Chapter 5). DNA samples are amplified and genotyped with 8 STR markers in PowerPlex® 1.1

Figure 14.8

(left) PowerPlex 1.1 STR data collected from the 505 nm (fluorescein-labeled alleles) and 585 nm (TMR-labeled alleles) scans of a gel containing allelic ladders and PCR-amplified samples. (right) PowerPlex 2.1 STR data collected on the same two samples. The three marked loci - TH01, TPOX, and VWA - are common to both multiplex kits and are therefore useful as a quality assurance measure to demonstrate concordance from two separate PCR amplifications of the same DNA sample. Note that the allelic ladders for VWA and TH01 have different alleles for the PowerPlex 1.1 and 2.1 systems yet the samples shown here provide the same genotype and thus demonstrate full concordance of PCR amplifications from both kits. The 9.3 and 10 alleles for the PowerPlex 2.1 TH01 allelic ladder are resolved (see boxed area) indicating that single base resolution can be achieved in that portion of the gel electrophoretic separation. Original data courtesy of Hitachi Genetic Systems.

PowerPlex® 1.1

Fluorescein-labeled 505 nm scan ladders ladders samples

D13S317

D5S818

TPOX

Amelogenin H _ 1.

PowerPlex® 2.1 505 nm scan 585 nm scan

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