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FIGURE 3.1 Elementary steps involved in the binding of antigen in solution to the antibody covalently attached to the surface (dual-step binding). Involvement of lateral interactions between antibody-antigen complexes on the surface is also shown (fe3, fe„3).

Sadana, A. and Madugula, A., Biotechnology Progress, 9, 259-266 (1993). Reprinted with permission from Academic Press.

given by

The reaction schemes showing lateral interactions may be lumped together as and

With the inclusion of the lateral step, the lumped reaction scheme is fe};(Ab • Ag)

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