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FIGURE 7.3 Binding and dissociation of different analytes in solution to receptors immobilized on a surface plasmon resonance biosensor (Nilsson et ai, 1995) (a) T7 DNA polymerase/ complementary DNA fragment; (b) DNA polymerase I (Klenow fragment)/complementary fragment; (c) endonuclease XhoI/69 bp substrate complementary DNA. (—, single-fractal analysis; —, dual-fractal analysis).

TABLE 7.2 Fractal Dimensions and Binding and Dissociation Rate Coefficients Using a Single- and a Dual-Fractal Analysis for the Binding and Dissociation of Different Analytes in Solution to Receptors Immobilized on a Surface Plasmon Resonance (SPR) Biosensor Surface During DNA Manipulations (Nilsson et al., 1995)

TABLE 7.2 Fractal Dimensions and Binding and Dissociation Rate Coefficients Using a Single- and a Dual-Fractal Analysis for the Binding and Dissociation of Different Analytes in Solution to Receptors Immobilized on a Surface Plasmon Resonance (SPR) Biosensor Surface During DNA Manipulations (Nilsson et al., 1995)

Analyte in solution/ receptor on surface

kbind

kl,bind

^2,bind

fediss

Cf.bind

Of i,bind

Dfj.bmd

Df.diss

T7 DNA polymerase/

455 ±69.6

201.2 ±40.8

1943 ±4.9

1517 ±44.9

2.19 ±0.13

1.76 ±0.38

2.75 ±0.01

2.73 ±0.03

complementary DNA fragment DNA polymerase I

250 ±31

106.2 ±9.3

1088 ±14.5

20.1 ±2.27

1.86 ±0.13

1.46 ±0.16

2.42 ±0.05

1.55 ±0.13

(Klenow fragment)/ complementary DNA fragment Endonuclease XhoI/69-bp

3556 ±193

na

na

0.049 ±0.01

2.89 ±0.049

na

na

0.496 ±0.14

substrate

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