Genetic Engineering Techniques

There are three general approaches that can be used to insert the DNA into a plant cell: vector-mediated transformation, particle-mediated transformation, and direct DNA absorption. With vector-mediated transformation, a plant cell is infected with a virus or bacterium that, as part of the infection process, inserts the DNA. The most commonly used vector is the crown-gall bacterium, Agrobacterium tumefaciens. With particle-mediated transformation (particle bombardment), using a tool referred to as a "gene gun," the DNA is carried into the cell by metal particles that have been acceler

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