Overview of Recombination Techniques

The basic technique of recombinant DNA involves digesting a vector DNA with a restriction enzyme, which is a molecular scissors that cuts DNA at specific sites. A DNA molecule from the organism of interest is also digested, in a separate tube, with the same restriction enzyme. The two DNAs are then mixed together and joined, this time using an enzyme called DNA ligase, to make an intact, double-stranded DNA molecule. This construct is vector carrier replication duplication of DNA

recombinant DNA DNA

formed by combining segments of DNA, usually from different types of organisms restriction enzyme an enzyme that cuts DNA at a particular sequence

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