Basic Procedure

In electrophoresis, an electric field is generated to separate charged molecules that are suspended in a matrix or gel support. Negatively charged molecules move toward the anode, on one side of the gel, and positively charged molecules move toward the cathode, on the other side. The gel itself is a porous matrix, or meshwork, often made of carbohydrate chains. Molecules are pulled through the open spaces in the gel, but they are slowed down by the meshwork based on their differing properties.

andode positive pole cathode negative pole

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