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Carboxyl terminus

FIGURE 3-27 Cleaving proteins and sequencing and ordering the peptide fragments. First, the amino acid composition and amino-terminal residue of an intact sample are determined. Then any disulfide bonds are broken before fragmenting so that sequencing can proceed efficiently. In this example, there are only two Cys (C) residues and thus only one possibility for location of the disulfide bond. In polypeptides with three or more Cys residues, the position of disulfide bonds can be determined as described in the text. (The one-letter symbols for amino acids are given in Table 3-1.)

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