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Phosphoenolpyruvate

Yeast enolase (Mr 93,316) is a dimer with 436 amino acid residues per subunit. The enolase reaction illustrates one type of metal ion catalysis and provides an additional example of general acid-base catalysis and transitionstate stabilization. The reaction occurs in two steps (Fig. 6-23a). First, Lys345 acts as a general base catalyst,

Enolase

Enolase

Lys345 Glu

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