Ii

Fmoc

Amino acid residue

Amino acid residue

Amino acid 1 with «-amino group protected by Fmoc group

FIGURE 3-29 Chemical synthesis of a peptide on an insoluble polymer support.

Reactions © through (4) are necessary for the formation of each peptide bond. The 9-fluorenylmethoxycarbonyl (Fmoc) group (shaded blue) prevents unwanted reactions at the «-amino group of the residue (shaded red). Chemical synthesis proceeds from the carboxyl terminus to the amino terminus, the reverse of the direction of protein synthesis in vivo (Chapter 27).

Fmoc

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