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of its neighboring peak by a charge difference of 1 and a mass difference of 1 (1 proton). The mass of the protein can be determined from any two neighboring peaks. The measured m/z of one peak is

where M is the mass of the protein, n2 is the number of charges, and X is the mass of the added groups (protons in this case). Similarly for the neighboring peak,

We now have two unknowns (M and n2) and two equations. We can solve first for n2 and then for M:

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