Info

Myosin 200,000

b-Galactosidase 116,250

Glycogen phosphorylase b 97,400

Bovine serum albumin 66,200

Ovalbumin 45,000

Carbonic anhydrase 31,000

Soybean trypsin inhibitor 21,500

Lysozyme 14,400 ©

Mr Unknown standards protein

FIGURE 3-20 Estimating the molecular weight of a protein. The electrophoretic mobility of a protein on an SDS polyacrylamide gel is related to its molecular weight, Mr. (a) Standard proteins of known molecular weight are subjected to electrophoresis (lane 1). These marker proteins can be used to estimate the molecular weight of an unknown protein (lane 2). (b) A plot of log Mr of the marker proteins versus relative migration during electrophoresis is linear, which allows the molecular weight of the unknown protein to be read from the graph.

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