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® 19. Working with Kinetics Go to the Living Graphs for Chapter 6.

(a) Using the Living Graph for Equation 6-9, create a V versus [S] plot. Use Vmax = 100 ¡m s—1, and Km = 10 ¡m. How much does V0 increase when [S] is doubled, from 0.2 to 0.4 ¡m? What is V0 when [S] = 10 ¡m? How much does the V0 increase when [S] increases from 100 to 200 ¡m? Observe how the graph changes when the values for Vmax or Km are halved or doubled.

(b) Using the Living Graph for Equation 6-30 and the kinetic parameters in (a), create a plot in which both a and a' are 1.0. Now observe how the plot changes when a = 2.0; when a' = 3.0; and when a = 2.0 and a' = 3.0.

(c) Using the Living Graphs for Equation 6-30 and the Lineweaver-Burk equation in Box 6-1, create Lineweaver-Burk (double-reciprocal) plots for all the cases in (a) and (b). When a = 2.0, does the x intercept move to the right or to the left? If a = 2.0 and a' = 3.0, does the x intercept move to the right or to the left?

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