Nadh

His thioester. ┬ęThe newly formed NADH leaves the active site and is replaced by another NAD+ molecule. The bond between the acyl group and the thiol group of the enzyme has a very high standard free energy of hydrolysis. ┬ęThis bond undergoes phosphorolysis (attack by P|), releasing the acyl phosphate product, 1,3-bisphosphoglycerate. Formation of this product conserves much of the free energy liberated during oxidation of the aldehyde group of glyceraldehyde 3-phosphate.

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