Sh

cleave with trypsin; separate fragments; sequence V by Edman degradation cleave with cyanogen bromide; separate fragments; ^ sequence by Edman degradation establish sequence

Amino terminus

(t-1 gasmalik t-2 egaayhdfepidpr

@ DCVHSD

t-4 yliacgpmtk

@ EGAAYHDFEPIDPRGASM C-2 TKDCVHSD

(C-3 ALIKYLIACGPM

T-2 placed at amino terminus because it begins with E (Glu).

T-3 placed at carboxyl terminus because it does not end with R (Arg) or K (Lys).

Q overlaps with

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