FTIR Spectra Analysis

The spectra obtained were analyzed for specific regions that showed distinct differences between normal and infected cells. The main peaks considered were as follows.

This peak is attributed to N-type sugars. Our results showed a gradual shift in the position of this peak from 861 cm-1 in normal samples to about 854 cm-1 in correspondence with the development of herpes viruses infection (29). For retrovirus-transformed cells, there was no shift in this peak.

Our data showed a gradual disappearance of the peak at 1023 cm-1 over time with the development of herpesviruses infection (29). This spectral peak can be attributed to carbohydrates, as was previously reported (31). There was also a notable decrease in this peak in cells infected with retroviruses (Fig. 1).

This peak is attributed to PO2 symmetric stretching vibration. A significant and detectable shift of the peak at 1080-1081 cm-1 for normal cells to 1086-1087 cm for retrovirus-transformed cells was observed (23), whereas there was no shift in this peak in cells infected with herpesviruses.

3.4.4. Peaks at 1200-1400 cm-1

The peaks in the region 1200-1400 cm-1 represent PO2- asymmetric stretching vibrations. Our results showed a statistically significant reduction in the intensity of the absorbance owing to these PO2- vibrations for retrovirus-trans-formed cells compared with normal cells (23) and a significant increase in the intensity of absorbance for cells infected with herpesviruses (28) (Fig. 2).

These differences may be considered key parameters for the detection of cells infected with these different viruses.

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