In recent years, the technique of small RNA interference (siRNA) for selective silencing of mammalian gene expression has rapidly developed (1-9). This technology can be a powerful tool not only for studying the molecular and cellular pathology of virus-induced diseases but also for the development of novel antiviral therapies (10-25). Here we use cervical carcinoma cells positive for

From: Methods in Molecular Biology, vol. 292: DNA Viruses: Methods and Protocols Edited by: P. M. Lieberman © Humana Press Inc., Totowa, NJ

Fig. 1. Restriction map of pSP64E6.

human papillomavirus type 16 (HPV16) to illustrate the method for selective silencing of viral gene E6 and E7 using antiviral siRNA.

0 0

Post a comment