Fig. 1. Identification of regions that are actively transcribed in the KSHV genome before and after induction with sodium butyrate in the presence of cycloheximide. (A) Summary of overlapping cosmid clones of KSHV DNA. Cosmids GB11, GA29, GB22, GA1, GA-2, and GB1 are referred to as cosmids 1, 2, 3, 4, 5, and 6, respectively. (B) Ethidium bromide-stained agarose gel (0.8%) of KSHV cosmid DNAs digested with £coRI, BamHI, or both as indicated. (C) Southern hybridization of restricted KSHV cosmid DNAs with cDNA probe prepared from uninduced BC-1 cells. (D) Southern hybridization of the restricted DNAs with cDNA probe prepared from sodium butyrate induced BC-1 cells. (Reproduced with permission from ref. 7.)

Fig. 1. (Continued)
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