Nancy M Sawtell


Herpes simplex virus (HSV), in contrast to most other members of the herpes virus family, has the ability to infect, enter latency, and reactivate from latency in a number of nonhuman species, including mice. This provides a unique opportunity to study the complex lytic-latent cycle of a human neurotropic virus in a mouse model. This chapter details basic methods for inducing and quantifying reactivation, with emphasis on the first strategy for detecting and quantifying the initiation of HSV reactivation in vivo.

Key Words: Herpes simplex virus; latent infection; persistent infection; viral reactivation; hyperthermic stress; initiation of reactivation; whole tissue in situ detection; viral latency; immunohistochemistry.

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