Vitaly Erukhimovitch Marina Talyshinsky Yelena Souprun and Mahmoud Huleihel

Summary

Fourier-transform infrared (FTIR) microscopy is considered a comprehensive and sensitive method for detection of molecular changes in cells. The advantage of FTIR microspectroscopy over conventional FTIR spectroscopy is that it facilitates inspection of restricted regions of a cell culture or a tissue. We have shown that it is possible to apply FTIR microscopy as a sensitive and effective assay for the detection of cells infected with various members of the herpes family of viruses and retroviruses. Detectable and significant spectral differences between normal and infected cells were evident at early stages of the infection. Impressive changes in several spec-troscopic parameters were seen in infected compared with uninfected cells. It seems that the change in spectral behavior is specific to the infecting virus, because cells infected with her-pesviruses showed different spectral changes compared with cells infected with retoviruses.

Key Words: Viruses; FTIR microscopy; malignant cells; retroviruses; herpesviruses; viral infection.

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