Antibodies Detection Of

Michael A Kerr, Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital Medical School, Dundee, Scotland

Copyright © 1998 Elsevier Ltd. All Rights Reserved.

The widespread use of diagnostic tests which depend on the use of antibodies, together with the increasing use of antibodies in research laboratories for the detection and quantitation of all manner of bio-chemicals, has resulted in the development of a vast array of different techniques which can be used to detect antibodies. The nature, abundance and purity of the antigen recognized by the antibody and the number of assays to be performed will be the main factors which determine the selection of one technique rather than another. The most commonly used techniques and their relative advantages and disadvantages are summarized below.

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