Detection of TGFp

To detect the involvement and quantitative changes of TGFp in experimental and clinical situations, either mRNA or total TGFp activity obtained after acid activation is measured. Bioassays for TGFp are based on inhibiting growth of mink lung cell line MV-l-Lu. In situ hybridization to detect TGFp, transcripts in, for example, blood mononuclear cells, is a useful approach. It bypasses many of the obstacles inherent to conventional determinations of the concentrations of circulating TGFp by, for example, ELISA. TGFp secreted around diseased regulation of cytokine mRNA, or post-transcrip-tional regulation of cytokine secretion. Translational control has been reported for TGF(3.

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