Erythrocyte Antibody Complement EAC rosette

The use of erythrocytes coated with complement components, especially C3, to form rosettes is an extensively utilized technique. However, other particles such as zyomosan or bacteria can also be used. There are a number of separate and distinct complement receptors on lymphocytes. Assays for CR1 receptor cells are performed using sheep erythrocytes sensitized with IgM antibody and purified complement components to build up a EACl-4b or EAC1-3b. Alternative methods using heat inactivated serum to prepare appropriate reagents are available. Detection methods for CR2 use the EA cells and whole serum to prepare an EAC3d. Reagents for the detection of CR3 utilize the EAC3b cell subsequently treated with P1H and C3 inactivator. In such assays purified isolated mononuclear cells are incubated with the appropriate reagent, enumerated in a hemo-cytometer, and the percentage of positive rosettes determined. These rosette methods can also be used to isolate populations of cells for additional experiments. These methods are inexpensive and allow an excellent yiled of cells. A summary of these tech niques and the mononuclear cell populations so identified is presented in Table 1.

See also: CD2; CD22: Cell separation techniques;

Complement receptors; Fc receptors.

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