Genetic engineering of antibodycombining sites

Site-directed mutagenesis experiments performed on an antilysozyme antibody showed that a double mutant VL Glu28—>Ser, VH Lys.56—►Gin increased 8-to 9-fold its affinity for the antigen. One interesting conclusion of this work was that removal of electrostatically charged side-chains and their replacement by noncharged residues with hydrogen bond potential allows formation of additional hydrogen bonds with the antigen and leads to an increase in affinity. Certainly, a prime consideration in the production of genetically engineered antibodies is an improvement in affinity for antigen. However, important questions are yet to be answered in this regard: 1) what are the structural mechanisms which lead to the increase in affinity, and 2) why don't the natural diversification mechanisms produce these same hypervariable sequences?

See also: Affinity; Antibodies, specificity; Antibody-antigen intermolecular forces; Immunoglobulin structure; Antibody-antigen complexes, three-dimensional structures.

How To Bolster Your Immune System

How To Bolster Your Immune System

All Natural Immune Boosters Proven To Fight Infection, Disease And More. Discover A Natural, Safe Effective Way To Boost Your Immune System Using Ingredients From Your Kitchen Cupboard. The only common sense, no holds barred guide to hit the market today no gimmicks, no pills, just old fashioned common sense remedies to cure colds, influenza, viral infections and more.

Get My Free Audio Book


Post a comment