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Figure 2 Isoelectric focusing in polyacryla-mide gel of a number of batches (lanes 2-15) of clinical grade erythropoietin, showing consistency of production. All batches show an identical spectrotype and the IEF pattern matches that produced by the reference standard (lanes 1 and 16). The pH values at points measured on the gradient are shown on the vertical axis.

nique is that proteins will be focused on an ion-exchange column if there is a pH gradient formed along the length of the column, and they will be eluted from the column at a pH value near to their individual pis. A mixture of acids, bases and ampholytes are used to form the gradient and equipment and reagents for chromatofocusing are available commercially. The procedure has the advantages that it uses a robust solid column support and has high sample capacity.

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