Initiation of the secondary immune response in vitro

Cytokine application

Neither exogenous cytokines nor preprocessed antigen are obligatory for secondary immune responses in vitro (Figure 7). When applied, it is usually to study their influence on the immune response. These characteristics relate to the more efficient capacity of memory T lymphocytes to respond after antigenic stimulation, produce cytokines, and interact with B lymphocytes. The response is further assisted by the B lymphocytes presenting antigen to memory T lymphocytes.

Sctondniy lymphoid tugan (lymph node, spleen, tonsils)

Secondary lymphoid organ (lymph rvorfo. spleen. tonsils)

Percherai blood

Collagftnase.'DNAaso digestion

Collagenflso/ DNAase digestion

FlcoH-Oialrcaale density centrtfugaVon

FlcoH-Oialrcaale density centrtfugaVon

Mononuclear «I suspension imrtioted mononuclear cells or

Adherent monocytes or

Low density dendritic cells

Mononuclear «I suspension

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