Employing transfection of cells with mutagenized plasmids or human CD2 chimeric and mutant proteins, distinct CD2 epitopes are defined by monoclonal antibodies and mapped to one of the Ig-like domains of the CD2 ectodomain. CD2 binds to the ubiquitously expressed cell surface glycoprotein, CD58, which is present on many human cell types including antigen-presenting cells and endothelial cells. A sheep homolog of CD58 exists which binds to human CD2, providing the molecular explanation for the fortuitous observation that human T lymphocytes and sheep blood cells spontaneously form rosettes. A second structurally related ligand, CD48, binds to CD2 but with an affinity two orders of magnitude weaker than that of the CD2/CD58 interaction. CD59 is also thought to bind to CD2.

Interactions of CD2 with its ligands are mediated through the N-terminal membrane distal Ig-like domain. The precise role of the second or membrane-proximal Ig domain has not been elucidated in depth, although activation of T cells in response to paired mitogenic combinations of CD2-specific monoclonal antibodies requires that one monoclonal antibody is directed against an epitope (CD2R) within or closely related to this region.

How To Bolster Your Immune System

How To Bolster Your Immune System

All Natural Immune Boosters Proven To Fight Infection, Disease And More. Discover A Natural, Safe Effective Way To Boost Your Immune System Using Ingredients From Your Kitchen Cupboard. The only common sense, no holds barred guide to hit the market today no gimmicks, no pills, just old fashioned common sense remedies to cure colds, influenza, viral infections and more.

Get My Free Audio Book

Post a comment