Mpo

MPO catalyzes the H202-dependent formation of HOCl which reacts with 02 to form OH". Singlet oxygen (102) is formed when an unpaired valence electron of oxygen is raised to an orbital of higher energy with an inversion of spin. There are two forms of J02, delta and sigma. Delta 'O,, while having a lower energy above ground state than sigma '02, has a considerably longer lifespan (approximately 2 /as) and is the reactive form of '0_> in solution. It is a strong electrophile which reacts with compounds in areas of high electron density to form oxygenated products. A well-established mechanism for the formation of '02 is by reaction of H202 with OC1. Since both reactants are formed by neutrophils their interaction to form '02 has been proposed as follows:

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