Figure 1 Measurements of B cell recruitment, clonal expression and activation of antibody secretion: cellular PFC and linnoval (ELISA) assays generate different conclusions. Limiting dilution cultures of human blood or tonsil lymphocytes were stimulated with TNP-ovalbumin in the absence (■) or presence (□) of high-dose (100 p,g ovalbumin per 24 h) primed, purified T cells. Cultures were assayed by hemolytic plaque assay for the frequency of TNP-specific PFC precursor cells and for the clone size present in positive cultures. A TNP-specific ELISA was used to measure specific antibody accumulated in supernatants over the 1-week culture period.

m 'Optimal' response □ plus T cells

Assay :

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