The IL8 gene family and related molecules

Since the discovery of IL-8, more than 20 similar cytokines have been identified (Figure 1). These cytokines contains four cysteine residues at the well-conserved positions with a molecular mass of around 8 kDa and possess the ability to attract and activate various types of leukocytes. Hence, the term 'chemo-kines' was proposed for this novel class of cytokines.

Chemokines can be subdivided into two subclasses, depending on whether the first pair of cysteine residues are separated by one amino acid (CXC) or adjacent (CC). The genes for CXC and CC chemokines reside on human chromosome 4q 12-13 and 17qll-12, respectively. CC chemokine, platelet factor 4 and platelet basic protein genes consist of three exons and two introns, whereas the other CXC chemokine genes have an additional exon and intron. However, the similarity of the splice junction and of the sizes of the introns and exons suggest that they all stem from a common ancestral gene.

Amino Add Sequences of Chemokines

CXC Chemokines

CXC c c

IL-8 AVI .PI-ÍSA KKI J<C:Q(:IK'['Y.SK1MI [PKI-IKKI.IiVIHSC^I^'AN'-ri:!! VKI.SI)(;KljLK:l _IJPKI-:.\WVQIÍ VVKK.H.KRAFÍNS

GRO a ASVATELRCOCLQT LQG1HPKNIQSVNVKSPGPHCAQTEVIATLKN GRKACLNPASPIVKKIIEKMLNSDKSN

GRO|3 APLATELRCDCLQT -LQG1HLKNIQSVKVKSPGPHCAQTEVIATLKN-GQKÁCLNPASPMVKKIIEKMLKNGKSN

GRO y ASWTELRCQCLQT LQGIHLKNTQSVNVRSPGPHCAQTEVIATLKN GKKACLNPASPMVQKIIEKILNKGSTN

NAP-2 AELRCMCIKT TSGIHPKNIQSLEVIGKGTHCNuVEVIATLKD GRKICLDPDAPRIKKIVQKKLAGDESAD

ENA-78 AGPAAAVLRELRCVCLCK TQGVHPKMISNLQVFAIGPQCaKVEVVASLKN-GKEMlLDPEAPFLKKVIQKILDGGNKEN

PF4 E AEEDGDLQCLC V KT TSQV RPREITS LEVI KA GPFJCPT AQLIATLKN GRKKLDLQAPLYKKITKKLLES

IP 10 VPLSRrVRcjrCIjISNQPVNPRSLEKLEIIPASQFCF|RVEIIATMKKKGEKRI£pNPESKAlKNLLKAVSKEMSKRSP

CC Chemokines

MCAF/MCP-1 MCP2 MCP3

1-309 SKSMQVPFS F

MIP-ta, LD78 ASLAADTPTACCF MI P|3. Act2 APMGSDPPTACCFS

RANTES SPYSSDT TPC

human Eotaxin GPASV--FTTCCFN

gp Eotaxin HPG I PSACC

QPDAINAPVllccjYNKrNKKISVQRLASYRRnSffflcPKEAVIFKTIVAKl QPDSVSIPITCZFNVlNRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKEVj QPVGINTSTKFCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLD] CCFSFAEQEIPLRAILCY RNTSSICISNEGLIFKLKRGKEJ SY TSRQIPQNFIADYFE-TSSQCS fÍRGVIFLTKRSRQVC/ fT ARKLPRNFVVD YYE TSSrCSQpk V VFQTKRSKQVC, CI A Yl ARPLPR A EI KEY FY TSGKCKN PAVVFVTR KNR< .ANRKIPLQRLESYRRITSGKCPyNAVIFKTKLAKDICAl FRVTNKKISFQRLKSYKIITSSKCPlbTAIVFEIKPDKMIcl

|CAI}PKQKWVQDSMDELI)KQTQTPKT

JPKERWVRDSMKELDQIFQNLKP (CADPTQKWVQDFMKELDKKTQTPKL

:aldtvgwvqrerkmlrhcpskrk fPSEEWVQKYVSDLELSA 3PSESWVQEYVYDLELN (fCANPEKKWVREYINSLEMS il'KKKWVQDSMKYLDQKSPTPKP

Jdpkxxwvqdakkyldqisqxtkp

Figure 1 Comparison of amino acid sequences of chemokines. Amino acid sequences are aligned according to the positions of cysteines.

How To Bolster Your Immune System

How To Bolster Your Immune System

All Natural Immune Boosters Proven To Fight Infection, Disease And More. Discover A Natural, Safe Effective Way To Boost Your Immune System Using Ingredients From Your Kitchen Cupboard. The only common sense, no holds barred guide to hit the market today no gimmicks, no pills, just old fashioned common sense remedies to cure colds, influenza, viral infections and more.

Get My Free Audio Book


Post a comment