Protocol 61 Target preparation

Depending on the sample source, type and quantity, the genomic DNA isolation method can vary. Select the one optimal for each sample. Hotstar Taq master mix (Qiagen. Cat. no. 203445) Primers 15 mM forward and reverse primers in DEPC-treated water PCR product quantification and visualization Agilent DNA12000 labchip kit (Agilent. Cat. no. 5064-8231) T7 Megascript kit (Ambion, Inc. Austin, TX. Cat. no. 1334) Target labeling reagents and material Low T dNTP (5 mM dA, dG and dCTP, 2mM dTTP) 1mM...

Array construction and application of genomic microarrays

Large insert clones such as cosmids, bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs) are used typically for the construction of genomic DNA microarrays. DNA derived from these clones was originally prepared from large-scale cultures (3, 4), which, when expanded to the number of clones that are required to construct an array with a resolution of 1 Mb ( 3500 clones) or even a whole genome tiling path array ( 37000 clones) becomes a costly and time-consuming procedure....