Animal Model

Male F344/N rats from Taconic Laboratories, Inc., Germantown, NY were used in these studies. The animals were obtained 36 ± 3 days old, and were about 89 ± 3 days old at the start of the study. Rats were housed three per cage in 22 x 12.5 x 8 inch (l x w x h) polycarbonate cages (Lab Products, Inc., Seaford, DE) with polyester cage filters (Snow Filtration Co., Cincinnati, OH). In studies dealing with fed animals, it is especially important to keep track of the cage assignment of the single animals. We decided to perform this study with fed animals in order to be closer to a human physiologically relevant situation. One potential disadvantage of this study design is that variability is introduced due to differences in feeding between animals in the same cage, based on their social hierarchy.

The room temperature was between 71 and 75°F, with the humidity between 36% and 48%. The animals were fed ad libitum with irradiated NTP-2000 wafer feed (Ziegler Brothers, Gardners, PA) and had ad libitum access to city water (Durham, NC). The rats had a 12-h light period from 6 a.m. to 6 p.m. and a 12-h dark period from 6 p.m. to 6 a.m. The animals were dosed between 9 and 11 a.m.

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