Cdna Clones

We prepared a 7K RAFL cDNA microarray (10) to study the expression profiles of Arabidopsis genes under various conditions. The 7K RAFL cDNA microarray consists of about 7000 RAFL cDNA clones isolated from full-length cDNA libraries. It includes drought-and cold-inducible genes, responsive to dehydration (rd) and early responsive to dehydration (erd). A PCR-amplified fragment from a À control template (TX803; Takara, Kyoto, Japan) is used as an external control, and two cDNAs derived from the mouse nicotinic acetylcholine receptor epsilon-subunit (nAChRE) gene and the mouse glucocorticoid receptor homolog gene are included as negative controls.

0 0

Post a comment