Dna Sonication

1. Place the sonicator tip in a tube filled with distilled water.

2. Start sonication to clean the tip.

3. Dry the tip and place it in the tube containing the DNA sample. Sonicate the DNA sample.

4. Place the DNA sample on ice for at least 10 min.

5. Check fragment length of the sonicated DNA by agarose gel electrophoresis on a 1% 0.5 x TBE agarose gel.

6. The DNA must show a smear with estimated fragment size between 400 and 3000 kb. If not, repeat from step 3.

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