Hybridization procedures and parameters

Hybridization is the process of joining the complementary nucleic acid strands of the labeled target and the probes on the array. The minimum information about hybridization is:

• the labeled extract (sample) used in the hybridization;

• the array used in the hybridization;

• the solution (e.g. concentration of solutes);

• the amount of labeled extract used;

• time, concentration, volume, temperature;

• a description of the hybridization instruments;

• a detailed free-text protocol on how the hybridization was performed.

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