Cellular states are determined by the expression of thousands of genes. Thus, the transition between cell types, for example from a progenitor cell to a differentiated cell, involves changes in the activity of many genes. This transition is not a sudden event, but takes place over a certain time range. The microarray technology allows us to simultaneously measure expression patterns of thousands of genes. To identify gene expression changes underlying the differentiation of neural progenitor cells (NPCs), we performed a time course study using cDNA microarrays. This chapter also addresses certain technical aspects, such as the fast and automated amplification of cDNA probes for the generation of DNA microarrays and the problem of limited biological material.

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