1. 0.15 |g DNA and 60 |l 2.5 x Random Primers Solution are resuspended in water to a final volume of 130.5 |l.

2. The solution is denatured in a heat block for 10 min at 100°C, and immediately cooled on ice.

3. The following reagents are added on ice:

4. The reaction is incubated at 37°C overnight and stopped by adding 15 |l of stop buffer supplied in the kit.

5. Labeled nucleotides are removed from the DNA labeling reactions using microspin G50 columns according to the instructions of the suppliers.

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