1. Grind 100 mg of tissue in liquid nitrogen to a fine powder and transfer into an Eppendorf tube.

2. Add 1 ml Trizol and continue according to manufacturer's protocol.

3. Purify the RNA on RNeasy microspin columns according to manufacturer's protocol.

4. Quality control: dilute sample 1:100 in 10 mM TRIS (pH 7.5). Measure absorbance at 260 nm and 280 nm. The ratio abs260nm:abs280nm should be between 1.9 and 2.1. Run 1 ^g of RNA on a 1% agarose gel. Nuclear and plastid ribosomal RNA should be visible as distinct bands. Alternatively, the Agilent Bioanalyzer 2100 (lab-on-a-chip) microcapillary system can be used to assess RNA integrity. For best results use only non-degraded RNA of high purity.

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