Microarray hybridization ChIPonchip

Figure 14.1.

Diagram showing the different aspects that can be investigated with ChIP assays. A nucleosome is represented as a circle, histone tails appear as protruding curly lines and DNA is represented as a black line. Antibodies (represented as three-way black lines) can be directed against different histone modifications (a, b) or against a variety of nuclear factors (c). Immunoprecipitated DNA can be analyzed either by studying candidate gene sequences, for instance by PCR amplification with specific primers, or by hybridizing appropriate microarrays (ChIP-on-chip).

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