Probe Preparation

1. Dry the pellet for a maximum of 10 min. Do not overdry the pellets because this will hamper resuspension. Keep the tubes in the dark during this procedure to prevent fading of the Cy-dyes.

2. Resuspend the pellet in 7.9 |l of distilled water, 12.0 |l of yeast tRNA and 16.3 |l 20% SDS.

3. Dissolve the pellet in the dark for 15 min. Subject the probes to a visual inspection to ensure that the probe is fully dissolved.

4. Add 84.0 |l of Mastermix. Vortex carefully and spin down.

5. Denature the probes at 70°C in a water bath for 15 min. Spin down afterwards.

6. Prehybridize the probes in a water bath of 37°C for 30 min.

7. The probes are now ready for hybridization on the hybridization station.

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